Enhancing polyethylenimine's delivery of plasmid DNA into mammalian cells
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چکیده
منابع مشابه
Enhancing polyethylenimine's delivery of plasmid DNA into mammalian cells.
The effect of various chemical modifications of nitrogen atoms on the efficiency of polyethylenimines (PEIs) as synthetic vectors for the delivery of plasmid DNA into monkey kidney cells in vitro has been systematically investigated. The resultant structure-activity relationship has both provided mechanistic insights and led to PEI derivatives with markedly enhanced performance. For example, N-...
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objective: dna vaccines have been widely used to develop immunity against various pathogens including parasites and viruses. the potential of dna vaccine to induce an effective immune response is related to the expression levels of the encoded protein in eukaryotic cells. therefore, optimization of plasmid dna delivery system is a major concern in protein expression in order to make an efficien...
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Branched polyethylenimine (PEI) chains with an average molecular mass of 2 kDa (PEI2) have been covalently attached to gold nanoparticles (GNPs), and the potency of the resulting PEI2-GNPs conjugates as vectors for the delivery of plasmid DNA into monkey kidney (COS-7) cells in the presence of serum in vitro has been systematically investigated. The transfection efficiencies vary as a function ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2002
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.192581499